|Number of Reactions(Preps) per Kit||250|
|Number of preps||250|
Recommended for isolation of Viral RNA from various samples like fresh and frozen plasma, serum, nasopharyngeal swab, oropharyngeal swab, sputum, BAL in Viral Transport Medium and other body fluids. Introduction HiPurA® Viral RNA Purification Kit provide the fastest and easiest way to purify viral RNA for reliable use in amplification technologies. Viral RNA can be purified from plasma (treated with anticoagulant EDTA), serum, other body fluids, and infected tissues. Samples may be fresh or frozen, but if frozen, should not be thawed more than once. Repeated freeze–thawing of plasma samples will lead to reduced viral titers and should be avoided for optimal sensitivity. HiPurA® Viral RNA Purification Kit can be used for isolation of viral RNA from a wide variety of viruses, but performance may vary depending on virus type.
This kit carries out efficient extraction of viral RNA from wide range of viral strains like Influenza, Dengue, Chikungunya and viral pathogen of animals. Sample is first lysed under the highly denaturing conditions provided by Buffer HRL to inactivate RNases and to ensure isolation of intact viral RNA. When Carrier RNA is added to Elution Solution (RNase-free Water), it improves the binding of viral RNA to the HiElute Miniprep Spin Column especially in the case of low-titer samples, and limits possible degradation of the viral RNA due to any residual RNase activity. Elution The yield of RNA depends on the sample type and the number of cells in the sample. A single elution with 50-80µl of Elution Solution will provide sufficient RNA to carry out multiple amplification reactions. 2 Concentration, yield and purity of RNA Spectrophotometric analysis and agarose gel electry.